Diarrhegenic E. coli (DEC) belongs to the one of the sex pathotypes: Enteropathogenic E. coli (EPEC), Enterohemorrhagic (or Shiga toxin–producing) E. coli (EHEC or STEC), Enterotoxigenic E. coli (ETEC), Enteroinvasive E. coli (EIEC), Enteroaggregative E. coli (EAEC) and Diffusely adherent E. coli (DAEC). The risk increased upon acquisition of the β-lactamases like ESBL, AmpC, KPC and MBL. The current study aim to phenotypically investigation of different β-lactamases types among DEC. Fifty eight stool samples were collected from children undergo diarrhea and have fever (38˚C- 41C˚) with age between (3 months to 4 years). The methods of feeding (Breast feeding or artificial feeding) and geographic area were also recorded. Stool samples collected by transport swab with medium and cultured in the same day on nutrient agar plates.The results revealed high percentage of DEC isolation among stool samples collected from children artificial feeding 33 (78.6%), while 2 (16.7%) positive among breast feeding and this may be due to breast milk secretory IgA, oligosaccharades and lactoferrin have dynamic role preventing adhesion and augmenting the immune response against DEC. Antibiotic susceptibility results revealed high sensitivity of DEC toward amikacin, norfloxacin and netilimicin whereas showed high resistance for the others. For ESBL detection the results display approximate similarity for double disc synergy test(DDST) and ESBL Chromatic medium in which 19(48.7%) and 22(56.4%) positive for ESBL respectively. The positive results for carbapenemase resistant Enterobacteriacea (CRE) were (10.3%) using MIC strip and (15.4%) when the CRE chromatic medium used. AmpC positive among DEC were (7.7%) while (10.3%) of isolates were positive for MBL using MIC strip test. The current study conclude importance of the natural breast milk feeding for the children to get rid the intestinal pathogens especially diarrhegenic E. coli and possessing of diarrhegenic E. coli for different types of β-lactamase leads to difficulties in treatments.
Keywords: AmpC, Diarrhegenic E. Coli, ESBL, KPC, MBL
