Anti-bacterial activities of the leaf of V.amygdalina (bitter leaf) was tested on some microorganisms. Five concentrations 0.5g/ml, 1.0g/ml, 1.5g/m, 2.0g/ml and 2.5g/ml were used and the control experiment was carried out to compare the diameter zones or clearing from the extracts and already standardized antibiotics. Agar well plug method was used for the tests. The bitter leaf extract was made with cold water. Nutrient agar were prepared and inoculated with the different bacteria strains after which wells were made in the in the media and bitter leaf extracts were poured on them. The cold water extracts of V.amygdalina showed inhibitions on the five organisms according to concentration. The organism susceptibility varied with more inhibition to E.Coli, P.aeruginosa, Salmonella typhi, Klebsiella pneumoniae and least to Styaphalococcus aureus.
Keywords: Antibacterial, Bitter Leaf, Cold Water Extract, Micro Organisms
