Histomorphometric Effects of Sildenafil Citrate on the Testis of Normoglycaemic and Hyperglycaemic Adult Wistar Rats (Published)
Histo-morphometric effects of sildenafil citrate was investigated on the testes of adult Wistar rats. Twenty five male Wistar rats were weighed before and after the experiment and categorized into four treatment groups and one control group of five rats per group (n=5). They were treated with the following regimen for eight weeks duration. The control group (A) received feed mash and water ad libitum. Treatment groups B and C were the normoglycaemic groups and received low dose (1mg/kg body weight) and high dose (2mg/kg body weight) of sildenafil citrate, respectively. Groups D and E were the hyperglycaemic groups, treated with low dose (1 mg/kg body weight) and high dose (2 mg/kg body weight) of sildenafil citrate, respectively. Blood samples were collected for hormonal assay and the testes were excised and processed for morpholological changes.The results obtained showed significant (P<0.05) loss in both body and testicular weight in the hyperglycaemic groups, but insignificant (P>0.05) body weight gain in the normoglycaemic groups and insignificant (P>0.05) epididymal weight difference loss in all the treatment groups, compared to control. The hormone assay showed significant (P<0.05) difference in the levels of FSH, LH, testosterone and oestrogen in the hyperglycaemic groups compared to control. Testicular and epididymal tissues revealed mild distortions in hyperglycaemic treatments but with dose dependent improvement while the changes in the normoglycaemic treatments were essentially non-remarkable. The results suggest relative safety of sildenafil citrate in normoglycaemic and hyperglycaemic states, with beneficial testicular effects in the latter condition.
Keywords: Hyperglycaemia, Normoglycaemia, Sildenafil Citrate, Wistar rats, testis
Histo-Morphometric Effects of Sildenafil Citrate on the Testis of Normoglycaemic and Hyperglycaemic Adult Wistar Rats (Published)
Diabetes mellitus is a known cause of infertility and erectile dysfunction in men. Sildenafil citrate, used in the management of the latter, has been implicated of toxic role on the testis. This paradoxical impression is significant in diabetic men with erectile dysfunction that are of reproductive age. Histo-morphometric effects of sildenafil citrate was thus, investigated for eight weeks on the testes of twenty-five adult Wistar rats, weighed before and after the experiment and categorized into four treatment groups and one control group of five rats per group (n=5). The control group (A) received feed mash and water ad libitum. Treatment groups B and C (normoglycaemic) received low dose (1mg/kg body weight) and high dose (2mg/kg body weight) of sildenafil citrate, respectively. Groups D and E ( hyperglycaemic) were treated with low dose (1 mg/kg body weight) and high dose (2 mg/kg body weight) of sildenafil citrate, respectively. Blood samples were collected for hormonal assay and the testes were excised and processed for morpholological changes. The results showed significant (P<0.05) loss in body and testicular weight in the hyperglycaemic groups, but insignificant (P>0.05) body weight gain in the normoglycaemic groups and insignificant (P>0.05) epididymal weight difference in all the treatment groups, compared to control. The hormone assay showed significant (P<0.05) difference in the levels of FSH, LH, testosterone and oestrogen in the hyperglycaemic groups compared to control. Testicular and epididymal tissues revealed mild distortions in hyperglycaemic treatments but with dose dependent improvement, while the changes in the normoglycaemic treatments were essentially non-remarkable. The results suggest relative safety of sildenafil citrate in normoglycaemic and hyperglycaemic states, with dose-dependent beneficial testicular effects in the latter condition.
Keywords: Hyperglycaemia, Normoglycaemia, Sildenafil Citrate, Wistar rats, testis
Toxicity Impacts Of Silk Dye Waste on Sperm Parameters and Histopathology of Testis of Swiss Albino Male Mice Mus Musculus and Its Mitigation by Using Moringa Oleifera Leaf Extract (Published)
The environmental toxicology studies the effect of environmental toxicant on the health of organisms and on the different compartments of the environments. Different scientific studies have been carried out over the years on the toxic effect of the silk dyes waste effluents on organisms. But current study has been premeditated to evaluate the effect of Moringa oleifera (Lam) leaf extract on albino male mice Mus musculas which is subjected to silk dye waste. The sperm profile and histopathology of testis have been taken on accounts. Five sets of animals i.e. Group I (Control), Group II (fed with 50% silk dye), Group III (fed with 100% silk dye), Group IV (mice fed with 50% dye treated with M. oleifera leaves powder), Group V (mice fed with 100% dye treated with M. oleifera leaves powder) have been taken for experiment. The dose of silk dye was 2ml/day to both groups II and III and M. oleifera leaf is given as per the standard dose (300mg/kg b.w) to both animals of group IV and V. The results show that the M. oleifera leaf extract when fed to Gr IV and V mice, demonstrated the regeneration of germinal epithelial cells and basement membrane, significant mitigation of spermatogenesis in different stage of testis when compared with mice of Gr- II and III. Then the M. Oleifera leaf increased significantly number of sperm count and % of sperm motility but decrease the % of sperm mortality of mice when compared with Gr-II and III at 5% and 10% level of probability. This study suggested that the extract may have beneficial effect on histological sections of testis and sperm count, mortality and motility.
Keywords: Moringa Oleifera Leaf Powder, Silk Dye Waste, Sperm Parameters, Swiss Albino Male Mice, Toxicity Assessment, histopathology, testis