Treasury Single Account (TSA)’s prime purpose is to ensure accountability, improve transparency, and prevent abuse and mismanagement of public funds. Nigeria had to domesticate the law implementing TSA in 1999, however, the government ran a pilot scheme in 2012 to fully domesticate and observe the impact of such policy on the growing economy of the nation. Thus, this study aimed to examine the implications of TSA from its domestication, identify its benefits, examine the challenges and study its prospects considering the perspectives of the banking sector employees. The descriptive research design was adopted for the study using fifty (50) bank employees within Ondo State, Southwestern Nigeria who were randomly selected. Software for the Social Sciences Statistical System (SPSS) was used to obtain descriptive statistics such as central tendency, the measure of variability, kurtosis and skewness. The calculated values range from 2.60 to 3.88, 0.72 to 1.28, -0.08 to -2.20, -1.73 to 4.09 for mean, standard deviation, skewness and kurtosis respectively. All calculated p-values range between (.000 to .003), which are less than the level of significance of 0.05 (2-tailed). Hence, the null hypotheses are rejected while the alternate hypotheses are accepted. The strong relationship of up to 0.985 between the opinions of the respondents is a strong indication that the application of TSA has led to a reduction of monetary misappropriation and a drastic reduction of corrupt practices. Therefore, TSA should be implemented in every sector of the economy both public and private to ensure financial prudence, accountability, transparencies and as a tool in monitoring, expenses incurred.