Extraction of Dye Pigments from Different Endemic Plant Samples Using Various Extraction Methods (Published)
The study was carried out to explore the potentials of selected endemic plant extracts as alternative counter stain in bilological studies. Selected endemic plant samples were Rothmannia whitfieldiiand Penthacletra macrophylla, Lonchocarpus cyanescens Curcuma longa and Duranta repens. Preliminary qualitative and quantitative Phytochemical Screening Extraction were carried out using well-established laboratory protocols using different extraction techniques. The natural dye was extracted from the plant materials using Soxhlet, Cold water, Hot water and Ethanolic extraction methods and assayed on cytological, histological and mycological specimen. Results obtained showed that the extraction time varied with an increase in temperature and pH of plant yielding dyes. From all the solvents used for the extractions of dye extract from Lanchocarpus cyanesces, Duranta repens, Pentaclethra macrophylla and Curcuma longa), Soxhlet extraction method at 56.8 OC, 56.8 OC, 56.8 0C, 57.1, 56.8 and for two hours thirty minutes (2.30min) was observed to be the best solvent while 1% NaoH at 43.7 OC for less than an hour (0.45min) proves to be the best solvent for the extraction of Rothmania whitfieldii dye extract. The Cold-water extraction method is less effective to extract dye from the plant species as far as the time needed for extraction is concerned. Further study on the effects of the extracted dyes from Lanchocarpus cyanesces, Duranta repens, Pentaclethra macrophylla and Curcuma longa on cytological, histological and mycological specimen is recommended. The study has demonstrated the potential use of dyes from endemic plants as a suitable substitute for staining microorganisms in a very simple, cheap, easy and readily available procedure
Keywords: Duranta repens, Extraction, Lanchocarpus cyanesces, Pentaclethra macrophylla and Curcuma longa extraction methods, dye, endemic plant