Biofungicide are biologically pest (fungi) control agent and one of the solutions for sustaining agricultural output and environmental quality. In order to implement these environmental friendly biofungicide on disease causing fungi of crop plant effectively, it can be important to play attention to way of formulation and application. Biofungicide have many advantages over chemical fungicide like it is biodegradable, cheaper and harmful residues are not detected. In present study, two pathogenic fungi Fusarium oxysporum and Alternaria species were isolated from carnation(Dianthus caryophyllus) plant and anthurium leaves respectively. Bacillus bacterial strain was used to ascertain affectivity against these fungi Fusarium oxysporum and Alternaria species for antifungal activity. Both fungi growth were inhibited by this bacterial strain at different incubation period. Maximum inhibition percentage was found in Alternaria sp.than Fusarium oxysporum. Also it was made to evaluate the population dynamics of spawn based bacterial strain in different organic substrates so as to determine the shelf life of substrate based bioformulation of bacteria. There was a gradual decline in population in Bacillus bacteria in different carriers. After 90 days of storage maximum population of bacteria was observed in soil. Compost ranked 2nd talcum powder ranked 3rd but in case ash there was no growth after one month.
Keywords: Antifungal activity, Bioformulation, Biofungicide, Carriers, Fusarium oxysporum and Alternaria.sp
