Oil extract from Anise (Pimpinella anisum L.) was isolated and identified using several techniques such as thin layer chromatography, infrared spectrum, UV-spectrum and boiling point. The antibacterial activity of oil extract was determined against two different pathogenic bacteria such as Escherichia coli NCTC 5933and Staphylococcus aureus NCTC6571, the results reflect promising bacteria employed. The minimum inhibition concentration (MIC) of oil extracted was estimated against types of gram positive and gram negative were 10 and 2.5 µg/ml respectively.
Keywords: Anise, Antibacterial activity, Chromatography, Infrared spectrum., Oil extract
