The essential oil from the leaves of Eugenia astringens Cambess (OE-2) was obtained by hydrodistillation in a Clevenger apparatus modified. The essential oil chemical composition (0.17% yield) was analyzed in GC-MS. The main component in the leaves was the α – pinene. The result of the quantification of the OE-2 sample, the calibration curve showed that the percentage of α-pinene in the essential oil is 2.5% ± 3.9. Cytotoxic potential of the essential oil of Eugenia astringens Cambess was assessed by indirect diffusion in agarose gel in a strain of Staphylococcus aureus. The essential oil obtained in vitro antibacterial activity relevant for high dosage (25 µL). It has been found that the use of essential oil (12.5mL) along with the antibiotic (amoxicillin, 12,5 µL volume) did not potentiate the drug action, this remains invariable. The linearity of the calibration curve was proven in the evaluated concentration range, but through the linear model obtained by Ordinary Least Squares Method.
Keywords: ATCC8096., Cytotoxicity Test, Essential oil, Eugenia Astringens Cambess, Evaluation of Homocedasticity, GC-MS, Linearity, Staphylococcus Aureus
