Cell culture technology is fundamental to biomedical research, yet contamination remains a significant challenge, compromising experimental outcomes and incurring substantial costs. This review addresses key issues in current contamination management practices, including the need for customized laboratory-specific protocols, comprehensive economic assessments, and standardized recovery procedures. It evaluates methods to tackle these issues, such as automated monitoring systems, real-time contamination detection sensors, and AI-driven predictive models. Additionally, it underscores the importance of effective training programs for laboratory personnel and the value of longitudinal studies to assess the impact of low-level contamination on experimental results. By addressing these areas, the review aims to enhance the reliability and reproducibility of cell culture research and promote more effective and sustainable laboratory practices.
Keywords: Cell culture, contamination prevention, contamination recovery, decontamination techniques, economic impact, laboratory protocols
