Determination of viability in yeast cell culture for the study of some plant base insulin releasing and insulin like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum) (Published)
The determination of viability in yeast cell culture for the study of some insulin – releasing and insulin – like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum) was studied using standard techniques, and data analytical tools respectively. The yeast cell line was used for this study. Yeast cell measurement at OD600 was carried out with the assistance of a spectrophotometer. Yeast cell counting was done first with a hemocytometer to determine the yeast cell number (as a correlation between the OD600). The yeast cell was very viable 95.9%. The trypan blue dye exclusion and OD600 methods both suggest viability of the cells and effectiveness in interacting with the extract fractions as well as the glucose substrate. Single re-suspension, double re-suspension, first and second re-suspension for both re-suspensions, the YPDM with the cells and PBS for the, independent T-test and paired T-test gave similar result in each of the assays, in all significant results were found; suggesting the effectiveness of the trypan blue dye cell viability assay and the OD600 method were compared and verified. Therefore, the baker’s yeast (Saccharomyces cerevisiae) could further be recommended as the perfect eukaryotic microorganism for biochemical investigations.
Keywords: Abelmoschus esculentus L, Cell viability, Insulin-Like Therapy, Musa Paradisiaca, Yeast cell culture, and Dioscorea dumetorum
Nutritional Evaluation of Unripe Carica Papaya Unripe Musa Paradisiaca and Mucuna Cochichinensis, Weaning Food Formulation (Published)
Unripe Carica papaya, unripe Musa paradisiaca and Mucuna cochichinensis were processed into flour and formulated into diets MPPA, MPPB, MPPC, MPPD and MPPE used for preparing weaning food. The proximate composition, anti-nutritional factors, biological evaluation through feeding trial alongside a standard diet with nutrend infant formula was determined. Results shows that the formulated weaning diets MPPA, MPPB, MPPC, MPPD and MPPE had protein value of (16.50-20.50%), fat (2.89-4.02%) ash (2.47-2.84%) crude fibre (1.16-1.22%), moisture (9.60-9.80%) and carbohydrate (62.4-66.87%). The formulated diets have more protein than the control nutrend. Anti-nutritional values revealed oxalate (0.21- 0.24%), polyphenol (0.31-0.41%) trypsin inhibitor (27.6-43.8 tu/g), HCN (4.4-4.9mg/Kg), phytate (0.02-0.07%). Values were according to standard recommendation for complementary food. The biological evaluation of the formulated diets were significantly different (P<0.05) than that of the standard. Based on the performance of MPPB on the rats, it is recommended for use in household to prevent and restore normal health in children suffering from kwashiorkor and should be manufactured commercially by Food industries.
Keywords: Carica Papaya, Mucuna Cochichinensis, Musa Paradisiaca, Weaning Food