Determination of viability in yeast cell culture for the study of some plant base insulin releasing and insulin like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum) (Published)
The determination of viability in yeast cell culture for the study of some insulin – releasing and insulin – like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum) was studied using standard techniques, and data analytical tools respectively. The yeast cell line was used for this study. Yeast cell measurement at OD600 was carried out with the assistance of a spectrophotometer. Yeast cell counting was done first with a hemocytometer to determine the yeast cell number (as a correlation between the OD600). The yeast cell was very viable 95.9%. The trypan blue dye exclusion and OD600 methods both suggest viability of the cells and effectiveness in interacting with the extract fractions as well as the glucose substrate. Single re-suspension, double re-suspension, first and second re-suspension for both re-suspensions, the YPDM with the cells and PBS for the, independent T-test and paired T-test gave similar result in each of the assays, in all significant results were found; suggesting the effectiveness of the trypan blue dye cell viability assay and the OD600 method were compared and verified. Therefore, the baker’s yeast (Saccharomyces cerevisiae) could further be recommended as the perfect eukaryotic microorganism for biochemical investigations.
Keywords: Abelmoschus esculentus L, Cell viability, Insulin-Like Therapy, Musa Paradisiaca, Yeast cell culture, and Dioscorea dumetorum